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(A–D) PNN neuroprotective role in mitigating EBRO-induced alterations in levels of pro-inflammatory cytokines like TNF-α (A) , IL-1β (B) , IL-10 (C) in brain homogenates and TNF-α, IL-1β, and IL-10 in blood plasma (D) . To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: (A–D) PNN neuroprotective role in mitigating EBRO-induced alterations in levels of pro-inflammatory cytokines like TNF-α (A) , IL-1β (B) , IL-10 (C) in brain homogenates and TNF-α, IL-1β, and IL-10 in blood plasma (D) . To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Article Snippet: Also assessed neuroinflammatory cytokines TNF-alpha [KB1145; Krishgen Biosystem, Mumbai, India] and IL-1 Beta [KLR0119; Krishgen Biosystem, Mumbai, India] , and IL-10 [GENLISA, Krishgen, Maharashtra, India] ( ; ).

Techniques: Clinical Proteomics, Standard Deviation, Control